Publications
CellSpot™ has achieved a qualitative advance
over earlier methods of antibody screening by
integrating the past decade's two dominant trends
in assay design: multiplexing and miniaturization.
Multiplexing enables the discovery of higher quality
antibodies but the resulting clone frequency
is so low that miniaturization is needed make
screening cost effective. CellSpot™ enables screening
millions of individual antibody variants produced
by millions of individual antibody producing
cells.
These scientific publications describe current state-of-the-art antibody screening technologies.
- Multiplexing is a popular format for assay
of the pooled secreted product of >10,000
cells.
Braeckmans K, De Smedt
SC, Leblans M, Pauwels R, Demeester J (2002) “Encoding
microcarriers: present and
future technologies” Nature
Reviews: Drug Discovery 1:447-456
- SLAM (Single Lymphocyte Analysis Method),
provides miniaturization sufficient for generating
monoclonal antibodies from single lymphocytes.
Babcook JS, Leslie KB, Olsen
OA, Salmon RA, Schrader JW (1996) “A
novel strategy for generating monoclonal antibodies
from single, isolated lymphocytes producing
antibodies of defined specificities” Proc.
Natl. Acad. Sci. USA 93:7843-7848.
- Physical miniaturization using microscopic
wells has also been explored as a way to screen
millions of cells.
Love1 JC, Ronan JL, Grotenbreg
GM, van der Veen AG, Ploegh HL (2006) “A
microengraving method for rapid selection
of single cells producing antigen-specific
antibodies” Nature Biotechnology 24:703-707.
- An alternative approach to achieving breadth
of screening is to use genetically engineered
bacteria, each expressing a different antibody.
Hoogenboom HR (2005) “Selecting and screening
recombinant antibody libraries” Nature
Biotechnology 23:1105-1116.
- Recent advances in the handling of human B
lymphocytes ex vivo enables a comprehensive
survey of the natural human immune response.
Lanzavecchia A, Bernasconi N, Traggiai E, Ruprecht
CR, Corti D, Sallusto F (2006) “Understanding
and making use of human memory B cells” Immunological
Reviews 211: 303–309.
